CURRICULUM VITAE
PERSONAL RECORD:
Last Names Casart
Quintero
Firt Name Yveth
Birth Date 3 August,1970
Nationality Venezuelan
Phone: +58-212-5041712 / 5041455
FAX: +58-212-5041444
E-mail ycasart@ivic.ve; yvethcasart@yahoo.com
Address: Laboratorio de Biología Molecular,
Departamento de Biología Estructural,
Instituto Venezolano de Investigaciones
Científicas(IVIC).
Km 11 Panamericana. Edo.
Miranda.
Venezuela. Apdo. 20632.
Languages: Spanish
English(IELTS* Overall Band Score 7.0)
CURRENT POSICION: Research Fellow.
CURRENT/RECENT TOPICS:
-Chromosome
segregation in Mycobacterium tuberculosis.
-Regulation
of the cell cycle and dormancy in Mycobacterium tuberculosis.
-Pathogenesis
and immune reponse in the murine model of Tuberculosis induced by clinical
strains carrying IS6110 insertion
elements in the chromosomal origin of replication (oriC).
Research and Work Experience:
15 September, 2006-Actually. Research Fellow. Molecular Biology Laboratory, Department of Structural
Biology (DBE), Venezuelan Institute of Scientific Research (IVIC).
23 June,
2005-28 September, 2006. Postdoctoral
Fellow. Microbiology Laboratory. Institute for Glycomics,
22 November,2004-24
February, 2005. Visiting Scientist. Experimental Pathology Laboratory,
Department of Pathology. National Institute of Medical Sciences and
Nutrition “Salvador Zubirán”. México D.F., México. Supervisor: Dr. Rogelio
Hernández-Pando.
15 September 2003-15 September, 2006. Postdoctoral
Fellow. Molecular Biology
Laboratory, Department of Structural Biology (DBE), Venezuelan Institute
of Scientific Research (IVIC).
1 June,1996-14
May, 1997. Professional Research Assistant. Molecular Genetics Laboratory,
Microbiology and
15 April-31
May, 1996. Apprenticeship as Professional in Training. Molecular Genetics
Laboratory, Microbiology and
17 November-20
December 1993. Apprenticeship as Student in Training. Molecular Genetics
Laboratory, Microbiology and
August, 1993.
Tutorial as Visiting Student. Bufo marinus Project (Microbiology Group). Molecular Genetics Laboratory, Microbiology
and
1 January,
1991-30 June, 1994. Teacher Assistant at the General Biology Laboratory.
Department of Biology, Faculty of Sciences, Universidad de Los
TECHNIQUES:
General culture
methodology (E. coli, Mycobacteria
and Burkholderia pseudomallei) and safety considerations for Mycobacterium
tuberculosis and B. pseudomallei.
Isolation
and manipulation of Genomic/Plasmid/Cosmid DNA from Mycobacteria, B. pseudomallei and E. coli,
Cloning and
recombinant protein expression,
Electroporation,
Extraction
of RNA from Mycobacteria and E. coli,
Primer extension
analysis for transcription start-site mapping,
Northern,
Western and Southern blots analysis,
PCR, RT-PCR
and Real Time-PCR,
Transcriptional
fusions to gfp (green fluorescent
protein) and fluorescence measurement,
Bacterial
Two-Hybrid System,
b-galactosidase
enzyme assay,
Gene replacement
by homologous recombination,
Immunolabelling,
Fluorescence
and Brightfield microscopy, image adquisition and image analysis,
IS6110 Restriction
Fragment Length Polymorphism (IS6110-RFLP)
of Mycobacteria,
Protein-DNA
interactions analysis by EMSA and Footprinting with Hidroxil radical,
Chromatin
immunoprecipitation,
Bacterial
Two-component system-functional analysis,
Bacterial
Cell adherence and Invasion assays (B.
pseudomallei),
Computer: Windows, Word, Excel, PowerPoint, Adobe Photoshop, Image-Pro
Plus, MetaMorph.
EDUCATION:
April 1998-July 2003.
PhD in Microbiology, Instituto Venezolano de Investigaciones
Científicas (IVIC), Caracas, Venezuela.
1988-1996. Bachelor of Biology, Universidad de Los Andes
(ULA), Mérida, Edo. Mérida, Venezuela.
1987-1988. High School “John Jay”,
1983-1987. High School “Domingo Briceño”, Bobures, Edo.Zulia.
PUBLICATIONS:
1) Cimino
M.,
2) Turcios
L., Casart Y., Florez I.,
Jaspe R., Guerrero E., de Waard J., Salazar L.
Characterization of IS6110 insertions
in the dnaA-dnaN intergenic region
of Mycobacterium tuberculosis
sputum isolates. Clin. Microbiol. Infect. Accepted for publication.
3) Patiño
S.,
4) Casart Y., Turcios
L., Florez I., Jaspe R., Guerrero E., de Waard J., Aguirre D., Hérnandez-Pando
R., Salazar L. 2008. IS6110
insertion in oriC affects morphology
and growth of Mycobacterium tuberculosis
and attenuates virulence in mice. Tuberculosis(
j.tube.2008.03.006
5) Casart
Y., Gamero E., Rivera-Gutierrez S., Gonzalez-y-Merchand J., Salazar
L. 2008. par
genes in Mycobacterium bovis and
Mycobacterium smegmatis are arranged in
an operon driven by “SigGC” promoters. BMC Microbiology. 8:51, doi:10.1186/1471-2180-8-51.
6) Choi K.-H.,
Mima T., Casart Y., Rholl D., Kumar A., Beacham I.R., Schweizer H.
P. 2008. Genetics tools for select agent compliant manipulation of Burkholderia pseudomallei. Appl. and Environ.
Microbiol. 74(4):1064-1075 doi:10.1128/AEM.02430-07
7) Muñoz-Romo M., Sosa
M. and Casart Y. 2005. Digestibilidad
del polen de cactáceas columnares en los murciélagos glosofaginos Glossophaga
longirostris y Leptonycteris
curasoae (Chiroptera:Phyllostomidae.
Revista de Biología Tropical (Internacional Journal
of Tropical Biology and Conservation). 53(1-2):277
8) Salazar L., Guerrero E., Casart
Y., Turcios L. and Bartoli F. 2003. Transcription analysis
of the dnaA gene and oriC region of the chromosome of Mycobacterium smegmatis and Mycobacterium bovis BCG, and its regulation
by the DnaA protein. Microbiology. 149:773-784.
9) Arraíz N., Salazar
L., López G., Rodríguez R., Casart Y. y Takiff H. 2001. Caracterización
de la expresión y función de sM, un factor
sigma ECF en micobacterias. (Characterization of the expression and function of sM an ECF sigma factor in mycobacteria).Acta Científica
Venezolana. 52(Supl. 1): 40-41.
10) Rowland
B., Purkayastha A., Monserrat C., Casart Y., Takiff H. E. y McDonough
K. 1999. Fluorescence-based detection of lacZ reporter gene expression in intact and viable bacteria including
Mycobacterium species. FEMS Microbiology
Letters. 179:317-325.
SPECIALS WORK-COURSES:
25 October-5
November, 2004. HHMI International Course: “The Molecular Basis of Bacterial
Stress Responses”. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET).
8-27 September,
2000. EMBO Practical Course on
GFP and Advanced Microscopy in Cell Biology. Max Planck Institute for
Biophysical Chemistry.
18-22 June,
2000. Workshop: “Tools for Sequence Analysis” (Bioinformatics
in the Web). Centro Nacional de Cálculo Científico. Universidad de Los Andes. Mérida, Venezuela.
14-29 October,
1996. Theoretical and Practical
Course on Molecular Biology Applied to Tuberculosis. Biotechnology Institute,
CICV/INTA.
March, 1995.
Safe Handling of Open Radioactive Reagents Course. Human Resources
Management. Venezuelan Institute of Scientific Research (IVIC).
MEETINGS:
Casart Y.
Gómez K. and Salazar L. Cytolocalizatin of the chromosome partition protein
ParB in Mycobacterium tuberculosis
and interactions with other proteins. 6-9 July, 2008. 29th Annual
Congress of the European Society of Micobacteriology.
Patiño S., Alamo L., Cimino M., Casart Y., Bartoli
F., García M.J. and Salazar L. Detection of Mycobacterium tuberculosis using intricsic autofluorescence of the
bacilli. 6-9 July, 2008. 29th Annual Congress of the European
Society of Micobacteriology. Plovdiv, Bulgaria.
Turcios L., Casart Y., Flores
I., Jaspe R., Guerrero E., de Waard J.,
Aguirre D., Hernández-Pando R. and Salasar L. IS6110
insertion in the origin of chromosome replication of Mycobacterium tuberculosis affects bacilli morphology, growth and
attenuates virulence in mice. 3-6
October, 2007. XXIV Congreso Brasileño de Microbiología. XII Simposio Brasileño
de Micobacterias y II Encuentro de
C.-A. Logue,
Y. Casart, H. Schweizer, I. Peak and
L Salazar, L Turcios, Y Casart, J de Waard, J Gonzalez-y-Merchand,
D Aguilar and R Hérnandez-Pando. Effect of the high rate of
IS6110 transposition in oriC on the virulence of Mycobacterium tuberculosis. 2-7 April 2005.
Keystone Symposia: “Tuberculosis, Integrating Host and Pathogen Biology”. Whistler, Canada.
L Turcios, Y Casart, J de
Waard, L Salazar. Characterization of the dnaA-dnaN intergenic region of M.
tuberculosis from Venezuelan clinical isolates reveals recent IS6110 transposition in a short chromosome
region. 17-21
October, 2004. XVII Congreso Latinoamericano
de Microbiología y X Congreso Argentino de Microbiología. Asociación Latinoamericana
de Microbiología y Asociación Argentina de Microbiología. Buenos Aires,
Argentina.
Casart Y. and Salazar L. Niveles de expresión
de los genes parA y parB de acuerdo a la fase de crecimiento
en Mycobacterium smegmatis y Mycobacterium bovis BCG . 17-21
October, 2004. XVII Congreso Latinoamericano
de Microbiología y X Congreso Argentino de Microbiología. Asociación Latinoamericana
de Microbiología y Asociación Argentina de Microbiología. Buenos Aires,
Argentina.
Casart Y. and Salazar
L. Citolocalización de la proteína ParB de Mycobacterium tuberculosis.
17-20 November, 2003. XXII Congreso
Brasileño de Microbiología. Sociedad Brasileña de Microbiología. Florianópolis,
Brasil.
Casart Y. and Salazar
L. Micobacterial origen of replication segregation: Transcriptional analysis of the parAB genes of Mycobacterium bovis
BCG and M. smegmatis mc2155. 18-20 September, 2002.
IV Reunión del Grupo de Microbiología
Molecular de
Salazar L.,
Guerrero E., Casart Y., Turcios L. and Bartoli F. Transcription analysis
of the dnaA gene and oriC region of the chromosome of Mycobacterium smegmatis and Mycobacterium bovis BCG, and its regulation
by the DnaA protein. 18-20 September, 2002. IV Reunión del Grupo de Microbiología Molecular
de
Casart Y. and Salazar
L. Promoters identification in the jag-parB
region of Mycobacterium bovis
BCG. 50th AsoVAC Annual Convention. 19-24 November 2000. USB,
Salazar L.,
Arraiz N., Casart Y. and Takiff H. E. Expression of an M. smegmatis ECF sigma factor increases
both at high temperature and during stationary phase. ASM Conference on
“Tuberculosis: Past, Present and Future”. 8-12 July 1997.
Casart Y., Salazar
L. and Takiff H. E. Operon fusion of the DNA gyrase genes of M. tuberculosis
and lacZ. 45th AsoVAC
Annual Convention. November 1995. USB,
Sosa M.,
Muñoz M. and Casart Y. Columnar cacti pollen digestibility by the
glossophagine bats Glossophaga longirostris
and Leptonycteris curasoe. 43rd.
AsoVAC Annual Convention (AsoVAC: Venezuelan Association for the Scientific
Advance). November 1993. ULA,
AWARDS:
Member of the Researcher Promoter System from the Venezuelan
Foundation to Promote Research. Level
Member of
the Researcher Promoter System from the Venezuelan Foundation to Promote
Research. Level: Candidate (No.4287). http://www.ppi.org.ve. Ministry of
Science and Technology. Caracas, Venezuela. 27 July,2000 – 31 December 2003.
Student Performance
Award.
CUM-LAUDE
Honorable Mention. Faculty of Sciences. Universidad de Los Andes. Mérida, Venezuela.
22 March,
1996.
Referees:
1) Dr. Leira
Salazar, Head of the Molecular Biology Laboratory. Department of Structural Biology.
Instituto Venezolano de Investigaciones Cientificas (IVIC). Km 11 Carretera
Panamericana. Edo. Miranda, Venezuela.
Phone: +58-212-5041715.
Fax: +58-212-5041444.
E-mail: leiriasalazar@gmail.com; lsalazar@ivic.ve
2) Prof.
Ifor Beacham, Research Leader. Institute
for Glycomics,
Phone: +61-(0)7-555
28185. Fax: +61-(0)7-555 28098.
E-mail: i.beacham@griffith.edu.au
3) Dr. Rogelio Hernández-Pando, Head of the Experimental
Pathology Laboratory. Departament of Pathology. National
E-mail: rhdezpando@hotmail.com
4) Dr. Howard
Takiff, Head of the Molecular Genetics Laboratory. Centro de Microbiologia
y Biología Celular. Instituto Venezolano de Investigaciones Cientificas
(IVIC). Km 11 Carretera Panamericana. Edo. Miranda, Venezuela. Phone: +58-212-5041439.
Fax: +58-212-5041382.
E-mail: htakiff@ivic.ve
5) Dr. Carlos Martín Montañes, Profesor
Titular. Universidad de Zaragoza, Departamento de Microbiología, Medicina
Preventiva y Salud Pública. Facultad de Medicina. C/Domingo Miral, s/n.
50009 Zaragoza, España. Phone. +34-976-761759. Fax: +34-976-761664.
E-mail: carlos@posta.unizar.es