Leira A. Salazar F.
Personal Data
Place and date of birth: La Guaira, Venezuela. 17/12/1960
Nationality: Venezuelan
Marital Status: Common-law, two children (Oriele 23, Paul 5)
Present Position: Associate Full-Researcher
Work address:
Leira A. Salazar F.
Personal Data
Place and date of birth: La Guaira, Venezuela. 17/12/1960
Nationality: Venezuelan
Marital Status: Common-law, two children (Oriele 23, Paul 5)
Present Position: Associate Full-Researcher
Work address:
Molecular Biology Laboratory. Structural Biology Department. Venezuelan Institute for scientific Research.
Km 11 Carretera Panamericana. Altos de Pipe. Edo. Miranda.
Telephone: (58 212) 5041715. Fax: (58 212) 5041444.
or
Structural Biology Department. Venezuelan Institute for Scientific Research. 8424NW 56th street. Suite CCS00232. Miami FL 33166
Current address:
Urb. La Morita. Ruta 8. No. 154-B. San Antonio de los Altos. Estado Miranda. Venezuela. Telephone: (58 212) 3932709
E-mail: lsalazar@ivic.ve, leiriasalazar@gmail.com
Languages: Spanish, English (XX), French (XX)
Education
Biologist (1982-1987).
Central University of Venezuela. Faculty of Sciences. School of Biology. Caracas. Venezuela. Thesis supervisor: Dr. Vidal Rodriguez-Lemoine.
Master (1989-1990).
Recombinant DNA Technology. Faculty of Medicine. University of Cantabria. Santander. Spain. Thesis supervisor: Dr. J. Rodríguez.
PhD (1988-1993).
Doctorate in Cellular Biology. Central University of Venezuela. Faculty of Sciences. Caracas. Venezuela. Thesis supervisor: Dr. V. Rodriguez-Lemoine.
Additional coursework:
- Tuberculosis today. Central University of Venezuela. Venezuela. 1999- Chromosome Workshop. Guanacaste - Costa Rica. 1998- Nucleic Acid Synthesis and Gene Assembly. Centre for Genetic Engineering and Biotechnology. India. 1991
- Molecular genetic of yeast. Simón Bolivar University. Venezuela. 1990
- Studies in vitro of regulatory factors. Research Institute of Engineering and Molecular Biology (INGEBI). Buenos Aires – Argentina. 1988
Work Experience
· Associate Full-Research. Head of the Molecular Biology Laboratory. Structural Biology Department. Venezuelan Institute for Scientific Research. From September 2000 - present.
· Associate Full-Research. Molecular Genetic Laboratory. Centre of Microbiology and Cellular Biology. Venezuelan Institute for Scientific Research. September 1996 to September 2000.
· Post Doctoral position. Institüt Pasteur. Unité Génétique Moleculaire Bactérienne. September 1995 to August 1996. Supervisor: Dr. Stewart Cole
· Post Doctoral position. Molecular Genetic Laboratory. Centre of Microbiology and Cellular Biology. Venezuelan Institute for Scientific Research. June 1993 to August 1995. Supervisor: Dr. H. Takiff
Teaching experience
· Venezuelan Institute for Scientific Research. Professor of Microbiology and Basic Techniques in Molecular Biology. Coordinator. (1997-present)
· Coordinator of the graduate students program. (1997- 2007).
· Venezuelan Institute for Scientific Research. Professor and Organization of International Course “Advanced Techniques in Molecular Genetic of Mycobacteria”. Funded by The United Nations University. 3-15 October, 1999
· Venezuelan Institute for Scientific Research. Organization of Scientific Course “Bacterial Immunology” funded by FONACIT and teaching by R. Hérnandez-Pando and I. Estrada.
· Central University of Venezuela. Faculty of Sciences. School of Biology. Adjunct Assistant Professor of General Genetic and Microbiology laboratories. March 1991-February 1992
· Central University of Venezuela. Faculty of Sciences. School of Biology. Adjunct Assistant Professor of Microbiology laboratory. March-July 1989
Referees
Main Current Lines of Research
· Regulation of cell cycle proteins during dormancy
· Chromosome segregation and cellular division in Mycobacterium tuberculosis
· Effect of IS6110 insertions into the origin of chromosome replication of Mycobacterium tuberculosis
Grants
· European Union (2006-2008). Transcriptional Regulation and cellular localization of mycobacterial cell cycle proteins during dormancy. Contract LSHP-CT-2006-037566. Partners: R Manganelli (Italy), L Salazar (Venezuela), JM Reyrat (France), M Vicente (Spain), R. Hérnandez-Pando (Mexico).
Direction of Thesis
Undergraduate Students
· Sol Patiño. Biologist. Central University of Venezuela. 2003.
· Elba Guerrero. Biologist. Zulia University. 2001.
· Lizet García. Biologist. Central University of Venezuela. 1999.
· Ada Sánchez. Biologist. Central University of Venezuela. 1999.
· José D. Rosales. Biologist. Los Andes University. 1998.
· Mena Cimino. Biologist. Central University of Venezuela. 1994.
Graduate Students
· Mena Cimino. Doctor in Science. Venezuelan Institute for Scientific Research. 2006.
· Yveth Casart. Doctor in Science. Venezuelan Institute for Scientific Research. 2004.
· Lilia Turcios. Master in Biological Sciences. Simón Bolivar University. 2000
Currents Graduate Students
· Elba Guerrero. Doctor in Science. Venezuelan Institute for Scientific Research.
· Rossana Jaspe. Doctor in Science. Venezuelan Institute for Scientific Research.
· Oscar Feo. Doctor in Science. Venezuelan Institute for Scientific Research.
· Ingrid Flores. Master in Molecular Biology and Biotechnology. Pamplona University. Colombia.
· Sol Patiño. Master in Microbiology. Venezuelan Institute for Scientific Research.
· Paola Valero. Biologist. Zulia University.
Meeting Attended
· L Alamo, W Wriggers, A Pinto, F Bartoli, L Salazar, R Padrón. A new of frozen-hydrated tarantula myosin filament reveals a new intermolecular myosin interaction involved on myosin regulatory Light Chain phosporylation-mediated activation. Alpbach Workshop on Molecular Motors 2007. Alpbach-Austria. March 2007.
· M Cimino, L Alamo, M Granados, L Salazar. The structural maintenance of chromosome protein (SMC) of Mycobacterium tuberculosis is linked to the cytoplasm membrane and shows several localization patterns. XVIII Latin Amrican Congress of Microbiology. Chile. October 2006.
· L Salazar. Characterization of the mycobacterial autofluorescence and its use as a new diagnostic tool in the detection of tuberculosis. Improving Case Management and Control of Tuberculosis and Buruli Ulcer. Cotonou. Africa. December 2005.
· L Salazar, L Turcios, Y Casart, J de Waard, J Gonzalez-y-Merchand, D Aguilar, R Hérnandez-Pando. Effect of the high rate of IS6110 transposition in oriC on the virulence of Mycobacterium tuberculosis. Keystone Symposia. Canada. April 2005.
· L Turcios, Y Casart, J de Waard, L Salazar. Characterization of the dnaA-dnaN intergenic region of M. tuberculosis from Venezuelan clinical isolates reveals recent IS6110 transposition in a short chromosome region. XVII Latin American Congress of Microbiology. Argentina. October 2004.
· Y Casart, L Salazar. Levels of expression of the parA and parB genes through growth in Mycobacterium smegmatis and Mycobacterium bovis BCG. XVII Latin American Congress of Microbiology. Argentina. October 2004
· Y Casart, L Salazar. Cytolocalization of the ParB protein of Mycobacterium tuberculosis. XXII Brazilian Congress of Microbiology. November 2003
· Y Casart, L Salazar. Segregation of the origin of chromosome replication in mycobacteria: Transcriptional analysis of parA and parB genes of Mycobacterium bovis BCG and M. smegmatis mc2155. IV Meeting of the Molecular Microbiology Group. Spanish society of molecular microbiology. Santander-Spain. September 2002.
· L Salazar, E Guerrero, Y Casart, L Turcios, F Bartoli. Transcriptional analysis of dnaA gene and the oriC region of Mycobacterium smegmatis and Mycobactedrium bovis BCG, and its regulation by the DnaA protein. IV Meeting of the Molecular Microbiology Group. Spanish society of molecular microbiology. Santander-Spain. September 2002.
· L Salazar, E Guerrero. Transcription pattern of the dnaA-oriC region of M. tuberculosis and M. smegmatis. Workshop on Molecular Approaches to Tuberculosis. Madrid-Spain. December 2000.
· N Arraiz, H Takiff, L Salazar. Functional Characterization of SloA: a sigma factor involved in the extra cytoplasmic stress response in mycobacteria. XX Brazilian Congress of Microbiology. Brazil
· L Salazar, A Sánchez, L García, M Coello, Characterization of the dnaA gene of mycobacteria. de micobacterias. XX Brazilian Congress of Microbiology. Brazil
· L Salazar, N Arraíz, Y Casart, M Gomez, Takiff, H. Expression of a M. smegmatis sigma factor of the ECF family increases at high temperature and in stationary phase. ASM Conference on Tuberculosis: Past, Present & Future. Copper Mountain, Colorado. USA
· L Salazar, H Fsihi, C Rios, ST Cole, H Takiff. Chromosomal organization near the origin of DNA replication in Mycobacterium tuberculosis (Mtb), M. smegmatis (M.smeg) and M. leprae (M.lep). Keystone Symposia on Bacterial Chromosomes. New Mexico-USA. January .
· L Salazar, V Rodriguez-Lemoine. Nucleotide sequence of traJ gene of pSU233. 11th. European Meeting of Transformation Genetic in Bacteria. Budapest - Hungary August 1992.
· L Salazar, B Dorta, V Rodriguez-Lemoine. Microcine production from Salmonella typhimurium LT2 in continuous cultures. I Latin American Congress of Biotechnology. Argentina. 1987
· And other 27 Venezuelan meeting
Publications
Thesis
· Regulatory genes and sequences of the tra operon from the haemolytic plasmid IncFVII pSU233. Evolution of tra operon in F-type plasmids. en plásmidos tipo F. Doctor in Sciences. Central University of Venezuela. 1993.
· Analysis of regulatory region of the tra operon in the haemolytic plasmid pSU233-IncFVII. Master in Recombinant DNA technology. Cantabria University. 1989.
· Studies of the genes coding a microcin of Salmonella typhimurium LT2 (cepa M799-0). Licentiate in Biology. Central University of Venezuela. 1987.
Charter of book
· P del Portillo P, A Reyes, L Salazar, MC Menéndez, MJ García. Genomics and Proteomics. Chapter 2: The agent and interaction with the host. In: Tuberculosis. Amedeo Book Challenge. Edited by S. Leao, JC Palomino and V. Ritacco. In press
Journals
20*. Cimino M, Alamo L, Granados M, Casart Y, Salazar L. The structural maintenance of chromosome protein (SMC) is cytoplasmic membrane associated and displays a dynamic-helix arrangement in Mycobacterium tuberculosis. Manuscript in preparation.19*. Turcios L, Casart Y, Guerrero E, Jaspe R, Bartoli F, Flores I, de Waard J, Aguirre D, Hérnande-Pando R, Salazar L Natural IS6110 insertion in the origin of chromosome replication of Mycobacterium tuberculosis affect bacilli morphology and growth and attenuate virulence in mice. Manuscript in preparation.18*. Patiño S, Alamo L, Casart Y, Bartoli F, García MJ, Salazar L. Autofluorescence of mycobacteria in the detection of Mycobacterium tuberculosis. Manuscript in preparation17.* Casart Y, Gamero E, Rivera-Gutierrez S, Gonzalez-y-Merchand J, Salazar L. Mycobacterium bovis and Mycobacterium smegmatis par genes are arranged in an operon driven by “SigGC” promoters and are differentially expressed throughout their growth curve. Manuscript in preparation.16*. Cimino M, Alamo L, Salazar L (2006) Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy. BMC Microbiology 6: 3515*. Salazar L, Guerrero E, Casart Y, Turcios L, Bartoli F (2003) Transcription analysis of the dnaA gene and oriC of the chromsome of Mycobacterium smegmatis and Mycobacterium bovis and its regulation by the DnaA protein. Microbiology. 149, 785-79414. Cáceres AJ, Portillo R, Acosta H, Rosales D, Quiñones W, Avilan L, Salazar L, Dubourdieu M, Michels P, Concepción JL (2003) Molecular and biochemical characterization of hexoquinase from Trypanosoma cruzi. Molecular Biochemical Parasitology. 4091, 1-1213*. Salazar L (2001) Chromosome replication in mycobacteria. Acta Científica Venezolana. 52(1): 42-4412. Arráiz N, Salazar L, López G, Rodríguez R, Casart Y, Takiff H (2001) Characterization of a sigma factor ECF in micobacterias. Acta Científica Venezolana. 52(1): 40-4111*. Salazar L (2001) Cell cycle control in mycobacteria. Acta Científica Venezolana. 52(1): 19-2110*. Salazar L (2000) Inhibition of Chromosome Replication in Mycobacterium smegmatis: Effect of the rpmH-dnaA Promoter Region. Microbiology (UK). 146: 2199-2207.9. Fsihi H, de Rossi E, Salazar L, Cantoni R, Labo M, Riccardi G, Takiff HE, Cole ST (1996) Gene arrangement and sequence analysis of a 78 kilobase chromosomal fragment around the oriC origin of the human pathogen Mycobacterium leprae. Microbiology (UK) 142: 3147-31618. Salazar L, Fsihi H, de Rossi E, Riccardi G, Rios C, Cole ST, Takiff HE (1996) Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, M. leprae and M. tuberculosis and isolation of a functional origin from M. smegamtis. Molecular Microbiology 20: 283-293.7. Takiff HE, Cimino M, Musso MC, Weisbrod T, Martinez R, Delgado MB, Salazar L, Bloom BR, Jacobs Jr WR (1996) Efflux pump of the proton antiporter family confers low level fluoroquinolone resistance in M. smegmatis. Proceeding National Academy Sciences. 93: 362-366.6. Salazar L, Fsihi H, Rios C, Cole ST, Takiff H (1995) Chromosomal organization near the origin of DNA replication in Mycobacterium tuberculosis (Mtb), M. smegmatis (M.smeg) and M. leprae (M.lep). Journal of Cellular Biochemistry. Supplement 19A, pp 116.5. Takiff HE, Salazar L, Guerrero C, Philipp W, Huang WM, Kreisworth B, Cole ST. Jacobs WR, Telenti A (1994) Cloning and nucleotide sequence of the Mycobacterium tuberculosis gyrA and gyrB genes, and characterization of quinolone resistance mutations. Antimicrobial Agents and Chemotherapy 38: 773-780.4. Salazar L, Rodriguez-Lemoine V (1993) Nucleotide sequence of the pSU233 traJ gene. In DNA transfer and Gene of Microorganisms. pp 241-248. Edited by E. Balla, G. Berencsi and A. Szentirmai. Intercept Limited. Great Britain, UK3*. Salazar L, López J, Andrés I. Ortiz J, Rodriguez J (1992) Characterization and nucleotide sequence of the oriT-traM-finP region of the IncFVII plasmid pSU233. Molecular General Genetics. 234: 442-448.2. López J, Salazar L, Andrés I, Ortiz J, Rodriguez J (1991) Nucleotide sequence of the oriT-traM-finP region of the haemolytic plasmid pSU316. Nucleic Acids Research 19: 3451.1. Salazar L, Dorta B, Rodriguez-Lemoine V (1990) Growth rate and production of a microcin by Salmonella typhimurium LT2 (strain M799-0) in continuous culture. Microbios 61: 17-22.
*Correspondence author